Grapevine Biotechnology

Marker-free transformation by transient expression of regeneration-promoting factors

The marker-free transformation system uses different gene products that are able to transiently induce the regeneration potential of transferred cells, resulting in increased cell proliferation and formation of meristematic or embryogenic cells. Since these regenerator genes do not have to be integrated into the plant genome in order to function, the regenerator products function as transient selection markers in the production of transgenic regenerated plants. The resulting transgenic plants express stably integrated trait genes without integrated selection markers or vector DNA. The system is patented and was already successfully demonstrated by Genetwister Inc. for some ornamental plants where regeneration takes place by organogenesis. It is described in patent No. EP 1094113 A1. Genetwister Inc. has a set of regenerator genes (REG-genes) available that will be tested for their potential to induce regeneration processes in leaves of grapevine.

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